Biuret's solution shows a color change to which color in the presence of protein?

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Prepare for the PLTW Biomedical Science Test. Study with flashcards and multiple choice questions, each question comes with hints and explanations. Get ready for your exam!

Multiple Choice

Biuret's solution shows a color change to which color in the presence of protein?

Explanation:
Biuret testing detects peptide bonds in proteins. In strongly alkaline conditions, copper(II) ions from the reagent form a complex with these peptide bonds, producing a purple–violet color. The appearance of this violet/purple color indicates protein is present (the reagent alone is blue; without protein it stays blue). So the color change in the presence of protein is violet or purple.

Biuret testing detects peptide bonds in proteins. In strongly alkaline conditions, copper(II) ions from the reagent form a complex with these peptide bonds, producing a purple–violet color. The appearance of this violet/purple color indicates protein is present (the reagent alone is blue; without protein it stays blue). So the color change in the presence of protein is violet or purple.

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